RUS ENG

Grant of the Government of the Russian Federation for the state support of scientific researches, which are conducted under the guidance of leading scientists in Russian educational institutions of higher professional education (agreement No. 11.G34.31.0065 dated October 19, 2011)

Regeneration of airways and lung

The official website of the project "Investigating the molecular mechanisms and underlying pathways of regenerative medicine approaches the tissue-engineering and cell therapy of airways and lungs"

Main page

About the project

Events and announcements

Conferences and Schools

Publications

Protocol

The project partners

About Regenerative Medicine

Results

Contacts

Within 2 years and 2 months of implementation of the project an International research, clinical and educational center of regenerative medicine was built, completely equipped and started functioning. The equipment (52 items totally) includes the unique systems – bioreactors for decellarisation and recellularisation of the organs and tissues for small and large animals. The young researchers, chosen on a competitive basis, were trained at the leading research centers of Russia and Europe and then started their own projects. A number of important scientific results in an investigation of regeneration molecular mechanisms, including development of new organs decellularization and recellularization protocols, studies of main properties of various organs and tissues decellularized matrices, were obtained at the basis of the new Center. The following was developed: International research protocol “Tracheal transplantation” (protocol, clinical investigation brochure, case report form, material certificates), Technical conditions “Nanocomposite scaffold seeding with bone marrow autologous mononuclear cells”The obtained results are being transferred into clinic. 4 tracheal transplantations (including the world’s first laryngo-tracheal transplantation) were performed in the Regional clinical hospital 1, which is the clinical basis of the Center in the course of Grant implementation. All transplantations were carried out with the use of nanocomposite scaffolds, seeded with patient’s cells, isolated from bone marrow. All the preparing procedures, including seeding and scaffold testing, were performed by Grant researchers, who underwent trainings at Karolinska Institute.

Main results

  • Database of literary sources in the field of regenerative medicine (486 sources);
  • Analytic review of literature in the area of regenerative medicine, existing and described methods, as well as general guidelines, paradigm and challenges;
  • Comparative analysis and development of different approaches to determine optimum methods of decellularization of different tissue types and organs, cell isolation, different scaffolds (both natural and synthetic), determining optimum cell sources for respiratory organs TE, finding materials for creating tissue engineered organ scaffolds;
  • Analytic review of research methods of cell isolation, differentiation, proliferation, reproduction, cell lines creation, gene expression in the process of stem cell differentiation, interaction with different scaffolds (both natural and synthetic), tissue;
  • Methodological recommendations on carrying out of in-vitro experiments for the work with cell technologies;
  • Technical conditions: “Rat heart decellularization protocol”;
  • Technical conditions: “Rat brain tissue decellularization protocol”;
  • Technical conditions: “Rat trachea decellularization protocol”;
  • Technical task: “Development of bioengineered diaphfagm on the rat model”;
  • Technical task: “Development of bioengineered heart on the rat model”;
  • Technical conditions: “Rat lung decellularization protocol”;
  • Technical conditions: «Rat diaphragm recellularization protocol using cryosections»;
  • Technical conditions: «Rat diaphragm recellularization protocol using decellularized matrix»;
  • Technical conditions: «Rat heart recellularization protocol using cryosections»;
  • Technical conditions: «Rat native heart recellularization protocol»;
  • Technical conditions: «Rat lung decellularization protocol»;
  • Technical conditions: «Biomechanical investigation method of decellularized matrixes of various organs and tissues (heart, diaphragm, lungs) »;
  • Technical conditions: «Physiological characterization method for lung decellularization using bioreactor»;
  •  Preliminary NHP heart decellularization protocol;
  •  Preliminary NHP lung decellularization protocol;
  •  Preliminary NHP diaphfarm decellularization protocol;
  •  Protocol of MSCs isolation from rat bone marrow;
  • Protocol of adipogenic, osteogenic, chondrogenic differentiation under the circumstances of standart stimulation media;
  • Protocol of chondrogenesis in MSCs culture in vitro under the circumstances of stimulating growth factor solution;
  • Device: «Bioreactor for decellularization/recellularization of rat diaphragm»;
  • Document Protocol «Tracheal transplantation» (protocol, researcher brochure, informed consent, registration card, materials’ certificates, abstract from the protocol of the ethical comission);
  • Specification Standard operation «Seeding of nanocomposite scaffold with autologous mononuclear bone marrow cells»;
  • Technical documentation “Protocol of case management and description of the tracheal transplantation”;
  • Technical documentation “Protocol of case management and description of the laryngo-tracheal transplantation”;
  • Tracheal and laryngo-tracheal transplantations were performed 19,21June 2012, 8,9 August 2013;
  • Method of biomechanical evaluation of nonhuman primates decellularized lungsmatrix”;
  • “Method of biomechanical evaluation of nonhuman primates decellularized heart matrix”;
  • “Method of biomechanical evaluation of nonhuman primates decellularized diaphragm matrix”;
  • “Protocol of decellularization of nonhuman primates’ diaphragm”;
  • “Protocol of decellularization of nonhuman primates’ heart”;
  • “Protocol of decellularization of nonhuman primates’ lungs”;
  • “Monitoring system of bioengineered scaffold decellularization”;
  • “Protocol of isolation, culture, proliferative and adhesive properties of rats’ mesenchymal multipotent stromal stem cells (MMSCs), cell typing using selective markers”;
  • “Protocol of adipogenic, chondrogenic and osteogenetic differentiation of rats MMSCs”;
  • “Protocol of isolation, culture, proliferative and adhesive properties of nonhuman primates’ mesenchymal multipotent stromal stem cells (MMSCs), cell typing using selective markers”;
  • “Protocol of adipogenic, chondrogenic and osteogenetic differentiation of nonhuman primates’ MMSCs”;
  • “Protocol of static recellularization of nonhuman primates’ decellularized matrices of heart, lungs and diaphragm with allogeneic MMSCs with subsequent evaluation of their viability”;
  • “Protocol of rat lung recellularization using decellularized matrix in bioreactor”;
  • “Heterotopic transplantation (subcutaneous test) of decellularized diaphragm matrices in rats”;
  • “Heterotopic transplantation (subcutaneous test) of decellularized heart matrices in rats”;
  • “Heterotopic transplantation (subcutaneous test) of decellularized lungs matrices in rats”;
  • “Protocol of spirometry in small laboratory animals (rats) for evaluation of airways functional properties after tissue-engineered diaphragm transplantation”;
  • “Evaluation of biomechanical properties stability of synthetic matrices in recellularization”;
  • “Protocol of recellularization of synthetic scaffolds with rats MMSCs obtained from bone marrow”;
  • “Protocol of recellularization of synthetic scaffolds with human blood MNCs”;
  • “Protocol of verification of acute myocardial infarction in rats”;
  • “Protocol of simulating of acute myocardial infarction”;
  • “Protocol of cell viability evaluation on previously recellularized synthetic scaffolds”;
  • “Protocol of simulating of pulmonary hypertension in rats”;
  • “Procedure EIA IL – 1a”, “Procedure EIA IL –1b”, “Procedure EIA IL –4”,
  • “Procedure EIA IL –6”, “Procedure EIA IL –17A”, “Procedure EIATNF –a”,
  • “Procedure EIATNF –y”, “Procedure of  C-reactive protein EIA evaluation”.

2012 Kuban State Medical University. The official website of the project "Investigating the molecular mechanisms and underlying pathways of regenerative medicine approaches the tissue-engineering and cell therapy of airways and lungs"